Rafsanjan University of Medical Sciences, Iran
Title: Neuroprotective Effect of Iranian Brown Propolis on Ischemic Brain Damage in Mice: a Possible Anti-inflammatory Activity
Inflammation plays a key role in ischemic neuronal injury. Propolis is a polyphenol-rich hive product with a set of biological activities.The aim of this study was to investigatethe effect of Brown Propolis (BP) on stroke outcome and inflammatory response in a mouse model of permanent middle cerebral artery occlusion (MCAO).Experimentally, the water extracts of propolis (WEPs) were obtained from two regions of Iran. The chemical characterization and total polyphenol content were determined using GC-MS and Folin–Ciocalteu assay respectively.Sixty-six adult male mice were randomly divided into the surgical sham group, control group (treated with vehicle), and four groups of WEPs-treated animals. WEPs were administered at the doses of 100 and 200 (mg/kg, i.p.), during four different time points. TNF-α level, infarct volume and brain edema were measured48 h post stroke.Behavioral tests were evaluated 4 and 48 h after stroke.
Samples were not substantially different in concentration of the total polyphenol content. In all treatment groups, WEPs treatment resulted in significant reduction of the TNF-α level and the subsequent decrease in infarct volume and brain edema compared to the control group. Sensory-motor impairment and neurological deficits were also improved significantlyas well.
Our finding showed that brown propolis reduces ischemic brain damage perhaps by exerting a neuroprotective effect on the stroke-induced neuroinflammatory responses.