Theodor Bilharz Research Institute, Egypt
Manal Mortady is a Professor of Medicinal Chemistry at Theodor Bilharz Research Institute (TBRI) from 2017 to till now. She had done her PhD in Organic Chemistry at Cairo University. Manal is a member at Egyptian Society of Natural Toxins and also a member in other 9 societies. She published 30 research papers. She is editorial board member for International Journals of : The Public Science Framework and The Journal of Harmonized Research Publications.
Our study was aimed to evaluate the in vitro cytotoxic activity of methanolic, ethyl acetate extracts and pure isolates of P. eugenioides against brine shrimps and HepG-2 cell lines using MTT assay. Qualitative phytochemical screening was carried out to detect the phytochemical constituents in the methanol extract; as well as its total phenolic content (TPC), total antioxidant capacity (TAC) and total flavonoid content (TFC) for the tested extracts were performed. Furthermore, TFC of the plant extracts was ranged from 32.40 to 310.15 mg RE / g dry extract and TPC of the tested extracts was ranged from 212.59 to 840.73 mg gallic acid equivalent (GAE)/g dry extract. Moreover the total antioxidant capacity (TAC) was ranged from 185 to 567.33 mg ascorbic acid equivalent/g dry extract. The antioxidant activity was evaluated qualitatively using DPPH method, thus revealed that the butanol extract record the strongest and potent free radical scavenging one among the tested extracts with SC50 value of 26.38 μg/ml. Ten compounds were isolated from the n-butanol extract of P. eugenioides, they were identified as; 3-O-[α - L- arabinofuranosyl - (1→3) - α - L - rhamnopyranosyl (1→2) - α - L- arabinopyranosyl ] hederagenin) 1), 3 β - 4β - 15, 16, 22 - trihydroxy olean 12-en-17 oic acid (2), rutin (3), isoquercitrin (4), Eudesmine (5), (2S,3S,4E,6S,7R,8R,9S,11E,13S, 14S,15R)- 7,8,9,14,15-pentaacetoxy-3-(benzoyloxy)-6-hydroxyjatropha-4,11-diene (6), β- Amyrin (7), limonene (8), α pinene (9) and kanesulone A (10). Compounds 1, 2, 3 and 4 showed the promising free radical scavenging activity SC50 value of 1.28, 3.21, 5.40 and 7.09μg/ml, respectively. Also, compounds 1-10 were evaluated for their cytotoxic activity against brine shrimps (Artemia salina L.). In, conclusion P. eugenioides leaves showed a potent antioxidant & cytotoxic activities that guided to be useful for developing the plant for potential therapeutic liver cancer treatments.