Scientific Program

Day1
Day2

Day 1 : 2019-03-13

Keynote Forum

Mohamed Eddouks

Moulay Ismail University, Moroco

Keynote: Buxus Sempervirens L. improves lipid profile in diabetic rats

Time : 10:00- 10:45AM

Biography:

Mohamed Eddouks is Professor at Moulay Ismail University, Faculty of Sciences and Techniques Errachidia, Morocco. He is a researcher in Physiology and Pharmacology with a Master Degree in Metabolic and Molecular Endocrinology from University of Paris vi, a specialized certificate in Endocrine Pharmacology from University of Paris vii and PhD degrees in Physiology and Pharmacology from University of Liege, Belgium and Sidi Mohammed Ben Abdellah University, Fez, Morocco. After his post-doctoral fellowship at Department of Physiology, Faculty of Medicine of Montreal, Canada, he is working for the last 16 years on medicinal plants. His work on antidiabetic and antihypertensive plants is well recognized globally.His contribution to this field includes four international books and more than 80 peer-reviewed articles and book chapters of international repute. He is Guest Editor of American Journal of Pharmacology and Toxicology, Lead Guest Editor of an annual special issue published by Evidence-Based and Complementary Alternative Medicine, Regional Editor of the Following journals: American Journal of Food Technology, Journal of Applied Sciences, Trends in Applied Sciences Research and Singapore Journal of Scientific research, Member of Editorial Board of several international journals. He has been honored with the first Prize of Scientific Research in 2008 by the Moroccan Association of Research and Development and the first Prize of the Federation of Arab Scientific Councils in 2016. He has been the Dean of Polydisciplinary Faculty of Errachidia from 2008 to 2012.

Abstract:

Buxus Sempervirens L. is used in Morocco as a plant with some medicinal properties including treatment of diabetes and cardiovascular diseases. The objective of the study was to assess the effect of the aqueous extract of Buxus Sempervirens L. (Boxwood) leaves on the
blood lipid parameters including total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C) in normal and streptozotocin-induced diabetic rats. In addition, antioxidant activity of aqueous Buxus Sempervirens extract was evaluated by DPPH and FRAP assays. The results indicated a significant decrease in serum total cholesterol and LDL-C levels, while, no change was observed on triglycerides and VLDL-C values in diabetic rats. Lipid profile of normal animals did not reveal any modification. In addition the aqueous extract of Buxus Sempervirens exerted a significant antioxidant activity. Therefore, the results suggest that the aqueous extract of Buxus Sempervirens exhibits lipid lowering activity which could be due partially to its antioxidant ability. In conclusion, Buxus Sempervirens leaf extract may be helpful in controlling the development of hyperlipidemia as well as atherosclerosis in diabetic animals.

Keynote Forum

Prem Mathi Maran

CEO, Clean Green Biosystems, India

Keynote: A Noval method of extracting highly bio available Gallate catechins from fresh tea leaves- An Anti malarial Drug

Time : 10:45- 11:30AM

Biography:

Dr. Prem, the founder Director and CEO of Clean Green Biosystems, Clean Green Bio Research Foundation and CRS Bio Engineering Pvt Ltd. Dr.S.Prem Mathi Maran, completed his Masters degree in Biology, Master of Philosophy in Toxicology and Doctor of Philosophy in Bio Chemistry. He is due to get his Doctor of Science in Phytochemistry. Dr.Prem is having 18 years of experience in the phytochemicals Industry. He is expert in deriving the process for herbal extraction system, phytochemicals extraction system, nutraceuticals extraction system, natural food colour extraction system and fruits and vegetable processing system. He is having expertise in the process design, process development, method development of herbal/phytochemicals/nutraceuticals/functional foods extraction system. He has developed novel methods of extracting bioactive principles from fresh herbs , fruits and vegetables. Dr. Prems other areas of expertise in HPLC and HPTLC analysis of phytochemicals, Process validation, Drug delivery system, pesticides formulation technology and biological assays. He has authored more than 25 research publications in peer reviewed journals of national and International origins. He is the associate editor of Journal of Pharmacognosy and Phytochemistry and Journal of Biotechnology and bio Engineering. He is visiting faculty in few Indian universities and handling subjects on phytochemicals extraction to M.Tech and M.S courses. Doctoral committee member in a Indian University. Technical adviser in various herbal extraction companies and phytopharmaceuticals company in India and overseas.

Abstract:

A new method , which is patented , is developed to enhance the bio availability of fresh green tea leaves to extract Gallate catechins, an anti malarial drug. The method involves use of a patented hydro dynamic system which converts the fresh tea leaves into a nano emulsion. This active catechins rich nano emulsion is filtered and clear emulsion is collected. This clear emulsion is subjected to Liquid-Liquid extraction and the catechins are isolated through a solvent phase. Then the solvent phase is dried in a molecular distillation unit and subsequently in a vacuum tray drier to get a green coloured powder. The HPLC analysis revealed that this extract is having all the 8 catechins in higher levels and its bio availability is increased to 10 fold as compared to the commercially available green tea extracts.

Natural compounds in Drug Discovery

Session Introduction

Azhar Rasul

Government College University Faisalabad, Pakistan

Title: Discovery and characterization of novel microtubule disrupter by MorphoBase and ChemProteoBase Profiling

Biography:

Dr. Azhar Rasul is an Assistant Professor at Faculty of Life Sciences, Government College University Faisalabad. He obtained PhD fellowship jointly awarded by Ministry of Education (MOE), Pakistan and China Scholarship Council (CSC), China and completed his Ph.D. in Cell Biology (Chemical Cancer Biology) from Northeast Normal University, China. He later received China postdoctoral fellowship in 2012, Japanese Society for Promotion of Science (JSPS) Postdoctoral fellowship in 2013, and subsequently Tokyo Biochemical Research Foundation (TBRF) fellowship in 2015. He has published over 45 peer-reviewed research articles with commulative imapct factor over 100 along with more than 1000 citations. He also has published two book chapters. He has received Young Scientist award by International Union of Biochemistry and Molecular Biology (IUBMB) for year 2018. He has presented several invited talks at National and International level. Three PhD and Eight MPhil students are currently working under his supervision. He has obtained several national and international research grants amounting > 20 Million PKRs. His lab is actively engaged in interdisciplinary research on novel tumor biomarkers, cancer-related health disparities, and identification of non-toxic anti-cancer compounds for various hallmarks of cancer (cancer stem cells, cancer cell metabolism, and tumor hypoxia) from

Abstract:

Abstract: In our pursuit of discovering novel simple small molecules that potently inhibit cancer cell growth, NPDepo library containing small molecules was screened. Here, we report a novel potent cytotoxic agent that displayed broad cytotoxic activity against panel of cell lines. In this study, target of small molecule was recognized by phenotypic profiling systems (MorphoBase and ChemProteoBase). These results were further validated by in vitro polymerization assays. Furthermore, small molecule inhibits the microtubule formation and induces G2/M phase cell cycle arrest, indicating that potential cytotoxic effects might be through tubulin polymerization inhibition. This novel scaffold can serve as templates for developing novel microtubule-targeted agents, overcoming the confines of existing microtubule-inhibiting drugs.

Natural compounds for Various Diseases

Session Introduction

Mihaela Cudalbeanu

University of Galati, Romania

Title: Enzyme Inhibitory Activities of Nymphaea alba fruit extracts

Biography:

Miss Mihaela Cudalbeanu has her expertise in evaluation and investigation of plant extracts. She is PhD students in chemistry and she has abilities in separations and chromatographic analysis of the different fractions obtained from the vegetal extracts. She has also competences in the analysis of bioactive compounds, antioxidant activity, antimicrobials and in cytotoxicity studies.

Abstract:

Statement of the Problem: In the developing countries the diabetes and also the hyperuricemia can cause many problems for people's health and has shown an increasing trend among the urban population. The studies published before reported that the alcoholic extracts of Nymphaea alba from the Danube Delta Biosphere Reserve have bioactive compounds like polyphenols and flavonoids. The Nymphaea alba fruit extracts were investigated further to determine their therapeutic potential to inhibit key enzymes involved in carbohydrate metabolism or in the catabolism of purines. Methodology & Theoretical Orientation: The evaluation of enzyme inhibitory activity of alcoholic extracts of Nymphaea alba fruits was carried out against α-amylase and xanthine oxidase. It is known that polyphenols readily complex with protein and as a result, often, there is an inhibition of enzyme activity. Enzyme inhibitory activities of the extracts from Nymphaea alba fruits were assayed in vitro. Findings: The results indicate that the evaluated alcoholic extracts were exerted higher inhibitory activity against both α-amylase and xanthine oxidase enzymes, having the highest xanthine oxidase inhibitory activity (IC50 = 160 µg/mL), and also α-amylase highest inhibitory activity (IC50 = 4 mg/mL). Conclusion & Significance: The obtained results suggest that the Nymphaea alba fruit extracts have the major components inhibiting the α-amylase and xanthine oxidase activity in vitro.

Ethnobotany & Ethnopharmacology

Session Introduction

Alex Boye

University of Cape Coast, Ghana

Title: Hypoglycaemic effect and possible mechanisms of action of Abrus precatorius leaf extract in stz/nicotinamide - induced hyperglycaemic rats

Biography:

Abstract:

Background: Abrus precatorius is used in folklore medicine in Ghana to treat diabetes mellitus and asthma.

Objective: The study investigated hypoglycaemic effect of Abrus precatorius leaf extract (APLE) and its possible mechanisms of action by using in vivo and in vitro models.

Method: Diabetes mellitus (DM) was experimentally established in normoglycaemic Wistar rats (16 weeks) by sequential injection of Streptozotocin (STZ)-citrate buffer (60 mg/kg ip) and nicotinamide (NIC)-saline solution (110 mg/kg ip). With the exception of the sham group (No STZ/NIC injection; normal saline, 5 ml/kg ip; baseline FBG = 6.48 mmol/L), rats with fasting blood glucose (FBG) stable between 28 – 32.5 mmol/L seven days post STZ/NIC injection were randomly re-assigned to the following groups: model (STZ/NIC-induced hyperglycemia in rats; no treatment), APLE (100, 200 and 400 mg/kg respectively po) and metformin (300 mg/kg po) and treated for 28 days. Bodyweight and FBG were measured on weekly basis. FBG was determined by using standard glucose test strips on blood obtained from tail-puncture. At the end of treatment rats were sacrificed under chloroform anesthesia, blood collected via cardiac puncture and brain, kidney, liver and pancreas harvested. While the pancreas was processed, sectioned and H&E-stained for histological examination, the kidney and liver were homogenized for assessment of total anti-oxidant capacity. Median cross-sectional area of the pancreatic islets of Langerhans was determined for each group. Total antioxidant capacity of plasma and DPPH scavenging ability were determined by using ascorbic acid as standard. Also, α-glucosidase inhibitory effect of APLE was assessed relative to acarbose standard.

Results: At the end of 28 days post STZ/NIC treatment, cumulatively APLE (100, 200 and 400 mg/kg respectively) significantly (P < 0.05) decreased the initial FBG by 55.22, 76.15 and 77.77 % respectively) compared to model (-1.04 %) and metformin (72.29 %) groups. APLE increased median cross-sectional area (x10⁶μm²) of pancreatic islets compared to that of model group. APLE produced dose-dependent decrease in immune cell infiltration relative to model group. Generally APLE (400 mg/kg; 5.21 ± 0.02 AscAE μg/mL) produced significant (P < 0.05) plasma ascorbic acid equivalent (AscAE μg/mL) TAC compared to model (4.06 ± 0.04 AscAE μg/mL) and metformin (4.87 ± 0.03 AscAE μg/mL) groups. APLE produced concentration-dependent inhibitory activity against α-glucosidase activity at an IC₅₀ of 33.89 ± 0.06 μg/mL comparable to that of acarbose standard at an IC₅₀ 37.10 ± 0.45 μg/mL. APLE produced concentration-dependent DPPH scavenging activity though lower in potency compared to ascorbic acid.

Conclusion: APLE produced hypoglycemic effect in STZ/NIC-induced hyperglycemic rats possibly mediated through inhibition of α-glucosidase activity; increased free radical scavenging and antioxidant activities as well as amelioration of pancreatic islet atrophy.

Rebecca Lazarou

Beyond Alcohol, UK

Title: Ethnopharmacological survey of Britain conducted with the help of Eden Project and Pukka Herbs

Biography:

Abstract:

Methodology: The main method of data collection was distributing an online survey, recruiting participants at Eden as well as at various events, conducting semi-structured interviews and reading wider literature including market research. Data was analysed and categorised in the following sections: demographics, uses of herbal medicines/ attitudes/ regulation, and growing and sourcing.

Results: Total responses for each question ranged between N=136 to N=408. Use of herbal medicines was fairly equal across varying socioeconomic status, and fairly equal across males and females though overall females had a more positive attitude towards herbal medicine. This was explained partially by sociological factors and by the boost in herbal products via the beauty industry. Results show that herbal medicines are popular, particularly amongst the 36-55 year old age group, most likely because of a higher income as well as more health conditions than millennials. However, millennials still showed a key interest for use in the future and so this could be an indicator of an increase in plant medicine use in Britain in the future. People mostly used herbs for minor-self limiting conditions such as sleep, wellbeing and digestion, though some people used them for more serious conditions and 42% agreeing they are effective for major conditions. Popular reasons for use included that plant medicines are natural, can have less side effects, are effective, as well as for some a changing relationship with conventional medicines and increased distrust in them. Largely people were not aware of the THR scheme and did not use sufficient means of quality control for the products they bought. Around a third of people grew their own plants for health care, though those who don’t already grow seem disinterested in engaging with horticulture. However encouraging horticulture would be very beneficial for public health and individuals, as it has shown to increase physical, mental and emotional wellbeing and has lead to a decrease in many ailments. This was explained by various biological theories such as the Biophilia Framework Theory which suggests that wellbeing is dependent on ones relationship with nature.

Conclusions and Future Research: There has been in recent years a cultural shift towards perceptions of herbal medicine in Britain, thus use has increased. Therefore, increased quality control and wider regulation is needed, and the public need to be made more aware the THR scheme as most are unaware of it. It would be very beneficial for public health to encourage gardening for people’s wellbeing. The main issues with the study were sample size, bias and differences in reliability of answers due to non-response. Future research that would be relevant include asking people directly about their relationship with conventional medicine and if this has affected their use of herbal medicines, more research on peoples use of herbal medicines for major conditions, more research on the benefits of horticulture and how to increase this activity, also research on herbal medicine regulation systems and how best to increase regulation and awareness of it in Britain. In addition, more scientific research on plants used for the most popular conditions herbal medicines are used for (sleep, overall wellbeing, digestion, immunity, stress and anxiety). Furthermore the cultural shift is interesting from a sociological perspective, and specifically why, how and who’s attitudes have changed over the years, and what has stimulated the growth in herbal product sales. Further investigation would be warranted, to also help predict the future trends and usage. Finally a survey to find out why people do not use herbal medicines in order to understand the perspective of others who are not in favour of their use.

Chemistry of Marine Natural Products

Session Introduction

Ghazal Taghizadeh Farahani

Islamic Azad University, Iran

Title: Improving the oil yield of Iranian Jatropha curcas seeds by optimizing ultrasound-assisted ethanolic extraction process using Box-Behnken statistical design

Biography:

Abstract:

An ultrasound-assisted solvent extraction (UASE) procedure has been optimized to speed up oil extraction from Jatropha curcas L. seeds by response surface methodology-Box-Behnken design (RSM-BBD). The preliminary study to determine the best solvent for extracting oil from J. curcas containing more than 60% oil revealed that ethanol had the highest efficiency among chloroform, acetone, n-hexane and methanol. Three independent variables including UASE time (10-60 min), UASE temperature (30-60°C) and ratio of ethanol to sample (15-45 v/w) were investigated to achieve the highest oil yield. RSM-BBD demonstrated that a second-order polynomial model can adequately develop with high R2 (0.9952) using multiple linear regression analysis. The linear effect of ethanol to sample ratio had the most significant (P<0.05) effect on yield value. The most suitable combination of variables for higher yield increase (61.94%) was UASE time of 51.69 min, UASE temperature of 44.57°C and ratio of ethanol to the sample of 44.78 v/w. At this optimal condition, the yield increased to 62.12%, which was close to the amount predicted by the model which was nearly 2.5x more than the obtained yield in the Soxhlet extraction procedure (25.63%). Gas chromatography-mass spectrometry (GC-MS) of the oils extracted under optimal conditions showed that the most dominant fatty acids were oleic (26.15%), linoelic (24.21%), palmitic (21.09%) and stearic (6.28%) acids, respectively.

Pharmacognosy and Phytochemistry

Session Introduction

Mohamed Eddouks

Moulay Ismail University, Morocco

Title: Effect of aqueous Corrigiola telephiifolia extract on glucose metabolism in normal and diabetic rats

Biography:

Abstract:

Introduction: Corrigiola telephiifolia Pourr, is a perennial species, woody distributed throughout the north of Africa. This plant is used in traditional Mediterranean preparations and has many traditional uses especially treatment of diabetes.

Aim of the study: the current research was carried out to evaluate the antidiabetic effect of aerial parts aqueous extract (APAE) of Corrigiola telephiifolia (C. telephiifolia) on both normal and streptozotocin(STZ)-induced diabetic rats treated at a dose of 5 mg/kg body weight for fifteen days. In addition, a preliminary pytochemical screening for various components was realized.

Results: Single oral administration of the APAE of C. telephiifolia (5mg/kg) showed no significant change in glycaemia of normal and STZ-induced diabetic rats. In contrast repeated oral administration of C. telephiifolia reduced blood glucose levels from 4.11±0.10mmol/L to 3.16±0.16 mmol/L (p<0.01) 15 days after administration in normal rats. Furthermore, blood glucose levels were decreased from 17.84±1.75mmol/L to 1.93±0.33 mmol/L (p<0.0001) in STZ diabetic rats after fifteen days of treatment. According to the oral glucose tolerance test, C. telephiifolia (5 mg/kg) was shown to prevent significantly the increase on blood glucose levels in normal treated rats 30 min after glucose administration when compared to the control group. Concerning the preliminary phytochemical screening of C. telephiifolia, several compunds have been found such as polyphenols, flavonoids, saponins, mucilage and terpenoids.

Conclusion: The results show that aqueous extract of C. telephiifolia possesses significant antihyperglycemic activity.

Day 2 : 2019-03-14

Keynote Forum

Prakash Kinthada

JNTU University, India

Keynote: Transition metal complexes/organometallic compounds as anticancer/anti HIV drugs or in pharmaceutical industry

Time :

Biography:

Abstract:

Cancer is a dreadful disease and any practical solution in combating this disease is of paramount importance to public health. Cancer patients have burdened by drug induced toxic side effects, and no turned to seek help from the complementary and alternative medicine hoping for a better cure. Research on Platinum based drugs and Non Platinum based drugs is a Multi-Million Dollar Industry in USA and there is every need to produce safe drugs for the cure of this monstrous disease. Flavonoids have a long history of use in traditional medicines in many cultures. The phytochemical, curcumin is one of the major dietary flavonoid, belonging to a group of flavonol, Curcumin is a natural polyphenol. It is highly potential molecule capable of preventing and treating various cancers. Various dietary chemo preventive agents, turmeric powder or its extract are broadly used as therapeutic preparations in Indian System of medicine. We provide a summarized synthesis and structural determination of Curcumin Oxime, Curcumin Thiosemicarbazone derivative of Gold (III) complex. The use of these analogs for prevention of cancer tumor progression and treatments of human malignancies. A pharmacologic agent for treating and/or preventing cancer, among other diseases and conditions, and particularly breast, prostate, and pancreatic cancer, in humans and animals. The novel pharmacologic agent is an isoflavonoid or isoflavonoid mimetic covalently attached to a cytotoxic pharmacophore that, preferably has the ability to conjugate with a metal salt to form a more potent metal complex, particularly a Au (III) complex and other complexes of Platinum, Palladium, Ruthenium, Copper etc.

My talk would mainly encompass different Transition Metal Complexes/Organometallic Compounds that are presently used as drugs, especially Anticancer and Anti-HIV drugs, apart from Anti-inflammatory, Antimicrobial, Antibacterial and diseases like Arthritis and Parkinson’s Disease etc. The talk would mainly focus on the use of Medicinal Chemistry and it’s application to Drug Design and Development in Pharmaceutical Industry , especially Transition Metal Complexes and Organometallic Compounds viz. Gold, Platinum, Palladium And Ruthenium apart from Copper, Cobalt, Iron, Nickel, Zinc, Cadmium etc.

The main emphasis of my talk would be on Different class of Ligands, their Schiff’s Bases and Transition Metal Complexes especially Au, Pt, Pd and Ru, with the main aim of designing, developing very novel small molecules, as possible and extremely potential candidates as Anti-cancer and Anti-HIV drugs. The talk would provide an overview of current programs being undertaken in our laboratories, especially focused on the development of potent ligands capable of recognizing Binding sites and diverse strategies employed by my group for elucidation of Anti-Cancer and Anti-HIV drug Leads to Circumvent the problem caused by Cis-Platin.

We have synthesized and characterized several phytochemicals from Traditional Medicinal Plants and isolated some phytochemicals and made the corresponding Oximes, Thiosemicarbazones and Substituted thiosemicarbazones as ligands and synthesized, characterized, structurally elucidated their Transition Metal Complexes especially with Gold, Platinum, Palladium, Ruthenium, Copper etc. and Studied their Anticancer Activity, Nuclease activity etc. and tested their potential as Anticancer Drugs.

The main aim of our extensive/preclinical Pharmaceutical development program is to investigate the use of these extremely novel small molecules-metal complexes/compounds of phytochemicals, flavanoids etc., which have very interesting structural features and properties and hence are excellent candidates as Anti-Cancer and Anti-HIV drugs .The main aim of our research is Design ,Development and Synthesis of Transition Metal Complexes/ Organometallic Compounds that would certainly help to bring this force of nature from BENCH to BEDSIDE and enhance Cancer Killing with less toxic effects and would certainly lead to initiation of clinical trials.

Natural Product Pharmacology Research

Session Introduction

Pardis Mohammadi pour

Isfahan University of Medical science, Iran

Title: Evaluation of pharmacological activity of Sinularia secondary metabolite, a systematic review

Biography:

Pardis Mohammadi pour received her pharm D. from the Joundi Shapour University of Medical Science and studying PhD from Isfahan University of Medical Science. Where she studying the isolation, structure elucidation and structure– activity relationships of biologically active marine natural products from marine soft coral (Sinularia sp.) under the guidance of Professors Ghannadian.

Abstract:

The order Sinularia is a large, diverse and ecologically important group of marine invertebrates. In this work, we present an overview of the most promising marine bioactive compounds isolated From Sinularia in the first decade of the 21st century, which may have applications in the therapy of human diseases. The present study also discusses future perspectives for the bio prospecting of new marine natural product that produced by this species group of marine invertebrates.

This study was systematic review and reviewed publications were identified through searches in Scopus, science direct, PubMed, Proquest, Ovid and also Wiley by using the search terms “sinularia”, “metabolite” and “pharmacology” .The search was completed through December 2017, and was limited to articles published in English. Relevant articles were identified based upon the expertise and clinical experience of the authors. This review covered 31 species of Sinularia and their secondary metabolites effects. The result categorized in different pharmacological effects including: cytotoxic, antitumor and anticancer, activity against antibiotic-resistant and antibacterial activity, anti-inflammatory, anti-neuroinflammatory, neuroprotective activity, antiviral, antialgeal, and antifouling effects. And inhibitory effect on HIV-1 reverse transcriptase and p56lck tyrosine kinase, HIF-2α Induced Gene Expression, the release of elastase by human neutrophils, the generation of the superoxide anion (that shown in table 1).Studies showed that the most of the species were collected from Taiwan (that shown in figure 1) and most of them had the cytotoxic effect (shown in figure 2.).

Finally, the review speculates sinularia secondary metabolite were potent bioactive compound that had a potential to introduce as lead compound in many diseases especially in cancer and anti-inflammatory and neuro-degenerative diseases.

Natural compounds for Various Diseases

Session Introduction

BRAHMI Mostapha

Dr Moulay-Tahar University, Algeria

Title: ANTIDEPRESSANT AND ANXIOLYTIC EFFECTS OF Mentha Spicata ESSENTIAL OIL AGAINST CHRONIC Co-EXPOSURE TO LEAD AND MANGANESE DURING DEVELOPMENT IN WISTAR RATS

Biography:

Brahmi Mustapha Ph.D. in biochemistry and experimental toxicology and member in the laboratory of biotoxicology, pharmacognosy and valorization of plants at the university of saida algerie

Abstract:

The objective of this study is to evaluate the changes induced by lead and manganese by using the neurobehavioral and biochemical approach in young rats during the gestation and lactation period. As well as the effectiveness of the essential oil of mint, to restore or not these effects by treating the previously intoxicated rats by a daily dose of intraperitoneal injection of 0.1 ml HE / kg during a period of 21 days . However, the extraction by hydro-distillation, made it possible to obtain an HE with a yield of 1.38%. Chronic exposure to lead at a concentration of 2.84 mg / ml and manganese at a concentration of 4.79 mg / ml during the developmental period resulted in a decrease in body weight of young rats (J21) and reduced brain weight compared to controls. However, the use of different techniques relating to behavior tests: the raised cross labyrinth test (EPM) and the open field clearly reveal that MnCl2 Pb intoxication causes a major depressive state and a locomotor hypoactivity with exploratory disability in young rats from intoxicated females during the developmental period compared to controls. This is explained by the deleterious effects of Pb and Mn on dopaminergic and serotoninergic transmission. In contrast, results from biochemical assays showed that lead and Mn induced hypocalcemia, hypomagnesemia, hypo albuminemia, hypercholesterolemia, decreased total iron, and increased ALP activity. Moreover, the administration of the essential oil of Mentha spicata allowed to observe a regain of body weight, a regulation of neurobehavioral disturbances and different biochemical parameters which justify the great importance of the mint in the traditional medicine and these therapeutic virtues.

SOLMAZ MASOUDI

AZARBAIJAN SHAHID MADANI UNIVERSIT, IRAN

Title: EXTRATION AND IDENTIFICATION OF THE ACTIVEINGREDIENTS OF THE PLANT RHUBARB (RHEUM RIBES)

Biography:

Solmaz masoudi completed his masterÊ¹s degree in phyto chemistry with the title of extracting and the active ingredients of rhubarb (Rheum ribes) she has been active in the field of extraction of active substances, as well as research on polymeric substrates in the field of drug release. She is studying in the field of shape memory polymer her Phd degree.

Abstract:

Rheum ribes L.is one of the wide rhubarb species belonging to polygonaceace. R.ribes is locally knowing as usgum or ucgum and grown mostly in eastern turkey, Lebanon and azarbaijan region of iran. Young shoots and petioles of R.ribes are used against diarrhea as well as stomachic and antiemetic while juice of some parts of the plant is used against hemorrhoids, measles, smallpox its fresh stems and petioles are consumed as vegetable, and stems are also eaten fresh, which are used as digestive and appetizer in bitlis, eastern turkey, while the roots are used to treat diabetes, hypertension, obesity, ulcer, diarrhea and expectorant. the air dried powdered stems and flowers of rheum ribes were sequentially extracted at room temperature with hexane, chloroform and methanol. separation by thin-layer chromatography was performed on silica gel 60 F₂₄₅plates and column chromatography. the extracts were evaporated under vacuum to dryness. Hexane extract of stems of rheum was isolated by acetone/pet-ether (2:9) solvent system to 11 fractions was isolated. Chloroform extract of stems by chloroform/methanol/ethyl acetate (3:1:1) to 8 fraction was isolated and methanol extract by methanol/ethyl acetate /H₂o/acid acetic (2:1:1:0.5) to 7 fractions was isolated. hexane extract of flowers by acetone/pet-ether (3:7) 10 fractions and chloroform extract of flowers by acetone/pet-ether (1:3) to 6 fractions and methanol extract of flowers to 6 fractions was isolated. fraction 1 of chloroform stem extract by ethyl acetate/chloroform (3:4) and fraction 1 of methanol flower extract by ethyl acetate/ acid acetic/ water/ methanol (5:0.5:1:4) were isolated. Tests were performed to identify compounds tannin, terpenoid, flavonoid, anthraquinoid, Saponoid, alkaloid, steroid of flower and stem rheum ribes L extracts. Purified compounds were identified by FT-IR spectroscopy and H¹NMR and C¹³ NMR.

Pharmacognosy and Phytochemistry

Session Introduction

Mohamed Eddouks

Moulay Ismail University, Morocco

Title: Antihyperglycemic effect of the aqueous extract of Pistachia atlantica leaves in normal and diabetic rats

Biography:

Abstract:

Background: Pistachia atlantica Desf, (Anacardiaceae) is an important source of essential oil and phenolic compounds justifying the use of its various parts in traditional medicine.

Objective: The present work aimed to evaluate the antidiabetic and the antioxidant activities of the aqueous extract of leaves of Pistachia atlantica.

Methods: The current study evaluated the effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of leaves of Pistachia atlantica (PALAE) on blood glucose levels in normal and streptozotocin-induced diabetic rats. Furthermore, its effect on glucose tolerance, histopathological examination of pancreas and liver in accordance with the Hematoxylin-Eosin method were carried out. Likewise the antioxidant activity of this extract was evaluated by the method of trapping of free radical 2, 2- diphenyl-1 picrylhydrazyl (DPPH).

Results: Single oral administration of PALAE reduced blood glucose levels in normal (p<0.05), and STZ diabetic rats (p<0.0001), 6 and 4 hours after administration respectively. Furthermore, this extract had an optimal effect (p<0.0001) in both normal and STZ diabetic rats at 15^th and 7^thdays of treatment respectively. It was also shown to prevent significantly the increase on blood glucose levels 120 min after glucose administration, in both normal (p<0.05), and diabetic (p<0.01) treated rats when compared to the control group. In addition, histopathological analysis highlighted the positive effect of Pistachia atlantica on pancreas and liver. Finally, inhibitory concentrations of 50% of free radicals (IC50) of Pistachia atlantica was equal to 4.85 ± 0.54 µg/ml, while the synthetic antioxidant (BHT) showed an IC50 of 22.67 ±2.17 µg /ml.

Conclucion: the study demonstrates the antihyperglycemic effect of the aqueous Pistachia atlantica extract in diabetic rats which should be mediated through the amelioration of the oxidative stress as well as an improvement in pancreas and liver histology.

Mohamed Eddouks

Moulay Ismail University, Morocco

Title: Evaluation of the anti-hypercholesterolemic and antioxidant activity of Mentha pulegium (L.) aqueous extract in normal and streptozotocin-induced diabetic rats

Biography:

Abstract:

OBJECTIVE: Mentha pulegium L., an aromatic plant belonging to Lamiaceae family, is widely used by local population against diabetes, hypertension and cardiovascular disorders. The present study aimed to evaluate the in vivo antihypercholesterolemic effect of aerial parts aqueous extract (A.P.A.E) of Mentha pulegium (M. pulegium) in normal and streptozotocin-induced diabetic rat. Additionally, the in vitro antioxidant activity of M. pulegium A.P.A.E has been estimated.

METHODS: The effect of M. pulegium (L.) A.P.A.E (20 mg of lyophilized A.P.A.E per kg body weight) on plasma lipid profile was investigated in normal and streptozotocin (STZ) diabetic rats (n=6) treated for 15 days with M. pulegium (L.) A.P.A.E oral administration. The antioxidant activity was evaluated using DPPH (1-1-diphenyl 2-picryl hydrazyl) radical scavenging activity .Estimation of total polyphenol contents in A.P.A.E of M. pulegium was determined with the Folin-Ciocalteu reagent by the method using gallic acid as a standard phenolic compound. Also, flavonoids were estimated according to the method based on the formation of a complex flavonoid-aluminium.

RESULTS: the results show that 15 days of M. pulegium A.P.A.E oral administration has alleviated hyperlipidemia in diabetic rats by lowering significantly (p<0.01) the plasma cholesterol levels without affecting the triglycerides (TG) levels significantly. However, no significant decrease in plasma cholesterol and plasma triglycerides has been shown in diabetic control rats. In addition, A.P.A.E oral administration has exerted some increasing activity on plasma HDL-c level (54.84 %), whereas the HDL-c level, in diabetic control rats, has significantly decreased (p<0.05). On the other hand, M. pulegium A.P.A.E showed a high amount of flavonoid (83.07 ± 0.58 mg EQ / g of extract) and phenolic compounds (239.08 ± 35.40 mg EAG/g of extract). Also, according to the DPPH radical scavenging activity, this aqueous extract has demonstrated a significant antioxidant activity.

CONCLUSION: we conclude that 15 days of M. pulegium A.P.A.E oral administration exhibited a significant antihypercholesterolemic effect and has also demonstrated a not negligent increase in HDL-c levels without affecting the triglycerides levels. Furthermore, A.P.A.E has exibited a potent antioxidant activity. Therefore, phytochemical compounds and antioxidant activity of M. pulegium A.P.A.E may be seemingly implicated in the antihypercholesterolemic effect demonstrated in this study.

Extraction of Natural Compounds

Session Introduction

Sanaz Sarabian Nejad

Azarbaijan Shahid Madani University, Iran

Title: Application of response surface methodology for the optimization of ultrasound assisted emulsification micro extraction of fatty acids from pomegranate seed

Biography:

Abstract:

Pomegranate seed oil (PSO) is extremely rich and nutritious oil, which may be used for internally or externally for its medicinal properties. Pomegranate seed oil contain high levels of anti-oxidants that fight free radicals, skin aging, and the oil also is a potent source of punicic. Because of these properties, pomegranate seed oil is used to heal, protect, moisturize, nature, irritated skin, bring elasticity back to the skin and other skin problems.

In this research, a simple and high efficient ultrasound assisted emulsification microextraction (UAEME) technique was applied for separation and preconcentration of fatty acids of pomegranate seed. The effects of different variables on the extraction efficiency were studied simultaneously using an experimental design. The variables of interest in the UAEME were time and temperature of extraction, ratio of extracting solvent volume to the weight of the plant, emulsification time, type and volume of the micro emulsification solvent. A Plackett–Burman design was performed for screening in order to determine the significant variables affecting the extraction efficiency. Then, the significant factors were optimized by a Box–Behnken design (BBD) and the response surface equations were derived. The chemical compositions of the UAEME extract were identified by GC–MS and determined by GC–FID after derivation. The main components of the extracts under optimum USEME conditions were punicic acid, linoleic acid, linolenic acid, stearic acid and palmitic acid.

Drugs Derived from Natural Products

Session Introduction

Aasaithambi Kalaiselvi

Sri Venkateswara College of Engineering, India

Title: Bioefficiency of Cinnamomum verum loaded niosomes and its microbicidal and mosquito larvicidal activity against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus (Diptera: Culicidae)

Biography:

Abstract:

Abstract— Emergences of mosquito vector-borne diseases are considered as a perpetual problem globally in tropical countries. The outbreak of several diseases such as chikungunya, zika virus infection and dengue fever has created a massive threat towards the living population. Frequent usage of synthetic insecticides like Dichloro Diphenyl Trichloroethane DDT eventually had its adverse harmful effects on humans as well as the environment. Since there are no perennial vaccines, prevention, treatment or drugs available for these pathogenic vectors, WHO is more concerned in eradicating their breeding sites effectively without any side effects on humans and environment by approaching plant-derived natural eco-friendly bio-insecticides. The aim of this study is to investigate the larvicidal potency of Cinnamomum verum essential oil (CEO) loaded niosomes. cholesterol and surfactant variants of Span 20, 60 and 80 were used in synthesizing CEO loaded niosomes using Transmembrane pH gradient method. The synthesized CEO loaded niosomes were characterized by Zeta potential, particle size, Fourier Transform Infrared Spectroscopy (FT-IR), GC-MS and SEM analysis to evaluate charge, size, functional properties, the composition of secondary metabolites and morphology. The Z-average size of the formed niosomes was 1870.84 nm and had good stability with zeta potential -85.3 meV. The entrapment efficiency of the CEO loaded niosomes was determined by UV-Visible Spectrophotometry. The bio-potency of CEO loaded niosomes was treated and assessed against gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria and fungi (Aspergillus fumigatus and Candida albicans) at various concentrations. The larvicidal activity was evaluated against II to IV instar larvae of Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus at various concentrations for 24 h. The mortality rate of LC50 and LC90 values were calculated. The results exhibited that CEO loaded niosomes have greater efficiency against mosquito larvicidal activity. The results suggest that niosomes could be used in various applications of biotechnology and drug delivery systems with greater stability by altering the drug of interest.